C‐C Chemokine Receptor Type 5 (CCR5)‐Mediated Docking of Transferred Tregs Protects Against Early Blood‐Brain Barrier Disruption After Stroke

نویسندگان

  • Peiying Li
  • Long Wang
  • Yuxi Zhou
  • Yu Gan
  • Wen Zhu
  • Yuguo Xia
  • Xiaoyan Jiang
  • Simon Watkins
  • Alberto Vazquez
  • Angus W. Thomson
  • Jun Chen
  • Weifeng Yu
  • Xiaoming Hu
چکیده

BACKGROUND Despite recent evidence demonstrating a potent protective effect of adoptively transferred regulatory T cells (Tregs) in ischemic stroke, the mechanism for Treg mobilization and activation in the ischemic brain is, remarkably, unknown. This study determines the role of C-C chemokine receptor type 5 (CCR5) in mediating the docking and activation of transferred Tregs in their protection of early blood-brain barrier disruption after stroke. METHODS AND RESULTS Adoptive transfer of CCR5-/- Tregs failed to reduce brain infarct or neurological deficits, indicating an indispensable role of CCR5 in Treg-afforded protection against cerebral ischemia. Two-photon live imaging demonstrated that CCR5 was critical for Treg docking at the injured vessel wall, where they interact with blood-borne neutrophils/macrophages after cerebral ischemic injury. CCR5 deficiency on donor Tregs deprived of their early protection against blood-brain barrier damage. Using flow cytometry, real-time polymerase chain reaction, and immunostaining, we confirmed that the expression of CCL5, a CCR5 ligand, was significantly elevated on the injured endothelium after cerebral ischemia, accompanied by CCR5 upregulation on circulating Tregs. In a Treg-endothelial cell coculture, CCR5 expression was induced on Tregs on their exposure to ischemia-injured endothelial cells. Furthermore, CCR5 induction on Tregs enhanced expression of the inhibitory molecule programmed death ligand 1, which in turn inhibited neutrophil-derived matrix metallopeptidase 9. CONCLUSIONS These results suggest that CCR5 is a critical molecule for Treg-mediated blood-brain barrier protection and a potential target to optimize Treg therapy for stroke.

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عنوان ژورنال:

دوره 6  شماره 

صفحات  -

تاریخ انتشار 2017